MicroSpin S-400 HR spin columns for DNA purification from unincorporated primers (32-mers) and nucleotides using spin-column chromatography in applications including desalting, buffer exchange, and primer removal.

• Accommodates 25 to 50μL for post-PCR clean-up prior to cloning or a second amplification reaction, or 25 to 100μL for all other applications
• Convenient: prepacked with Sephacryl™ S-400 HR pre-equilibrated in TE (Tris/EDTA) buffer
• Ready to use: requires less than 4 min from sample application to collection of purified product
• Tested in nickase, single- and double-stranded exonuclease, and RNase assays

MicroSpin S-400 HR spin columns rely on gel filtration for PCR clean-up. Good product yield and purity are obtained with sample volumes from 25-100µL, and from nanogram to milligram quantities of DNA.